Certified Tissue Bank Specialist 2025 Complete Practice Test

The correct choice emphasizes the necessity of controlled freezing rates during the cryopreservation of tissues. The AATB Standards for Tissue Banking specify that freezing must occur in a monitored and controlled manner to prevent ice crystal formation within the tissue. This is critical because ice crystals can cause cellular damage, reducing the viability and functionality of the preserved tissue upon thawing.

Controlled-rate freezing involves gradually lowering the temperature of the tissue, which allows for the gradual formation of ice inside the cells, minimizing the size of the ice crystals. This method is designed to improve the outcome of the cryopreservation process, enhancing the preservation of tissue structure and cellular integrity.

While other options such as organization-defined SOPs, time constraints, or the use of liquid nitrogen are relevant to tissue banking practices, they do not directly address the specific requirement of freezing tissues in a controlled manner that safeguards against crystallization. Therefore, the focus on a controlled freezing rate is paramount in ensuring the high quality of cryopreserved tissues.